Fig. 2

Silencing circHIPK2 inhibited oxidative stress and autophagy-dependent ferroptosis in H/R-treated cells. HCM cells were subjected into different groups: Control, sh-NC, sh-circHIPK2, H/R, H/R + sh-NC, H/R + sh-circHIPK2. A RT-qPCR detection of circHIPK2 expression. B CCK8 was used to detect cell viability. C Flow cytometry detection of cell apoptosis. D Levels of oxidative stress markers MDA and SOD were determined by ELISA. E The changes of inflammatory factors (IL-6, IL-1β, TNF-α) were detected by ELISA. F Western blot measured protein levels of autophagy markers LC3II/LC3I, Beclin1 and p62. G Western blot assessed protein levels of ferroptosis markers GPX4, FTH1 and ACSL4. H Iron level was tested by kit. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001