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Fig. 3 | Journal of Cardiothoracic Surgery

Fig. 3

From: CircHIPK2 recruits SRSF1 to increase TXNIP mRNA stability and promotes autophagy-dependent ferroptosis and apoptosis in myocardial ischemia-reperfusion injury

Fig. 3

CircHIPK2 recruited SRSF1 to target TXNIP and stabilized TXNIP mRNA expression. A StarBase predicted the binding site of SRSF1 and circHIPK2, and the binding site of SRSF1 and TXNIP. B RT-qPCR detection of TXNIP expression. C RNA-nucleosome separation assay detected location of circHIPK2 in HCM cells. D RT-qPCR detection of TXNIP expression. E RIP assay verified the binding of circHIPK2 and SRSF1 in HEK-293T cells. F The mRNA levels of SRSF1 and TXNIP were detected by RT-qPCR. G ChIP assay verified TXNIP promoter binding to SRSF1. H Actinomycin D was used to detect TXNIP mRNA stability. Data are the means ± SD for three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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Journal of Cardiothoracic Surgery

ISSN: 1749-8090

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