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Fig. 5 | Journal of Cardiothoracic Surgery

Fig. 5

From: CircHIPK2 recruits SRSF1 to increase TXNIP mRNA stability and promotes autophagy-dependent ferroptosis and apoptosis in myocardial ischemia-reperfusion injury

Fig. 5

Silencing circHIPK2 inhibited the damage of MIRI mouse model. Mice were subjected to different groups: sham, I/R, I/R + sh-NC, I/R + sh-circHIPK2. A circHIPK2 expression in mouse heart tissue was detected by RT-qPCR. B Evans blue and TTC combined staining of mouse heart tissue. C HE staining detected myocardial injury. D Levels of oxidative stress markers MDA and SOD were determined by ELISA. E Western blot measured protein levels of autophagy markers LC3II/LC3I, Beclin1 and p62. F Western blot assessed protein levels of ferroptosis markers GPX4, FTH1 and ACSL4. G Iron level was tested by kit. Error bars represent the mean ± SD. *P < 0.05, **P < 0.01. n = 5 mice/group

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Journal of Cardiothoracic Surgery

ISSN: 1749-8090

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